The PDB file format is a standard structural format used extensively in bioinformatics and structural biology to represent the three-dimensional structures of large biological molecules, such as proteins, nucleic acids, and complex assemblies. Developed by the Protein Data Bank, these text-based files contain atomic coordinates, secondary structure assignments, experimental metadata, and crystallographic or NMR data. Researchers and scientists rely on PDB files to visualize molecular structures, perform computational docking simulations, analyze protein-ligand interactions, and study molecular dynamics. Because of its human-readable structure, the format allows scientists to easily parse atom records using custom scripts or specialized molecular visualization software to gain insights into biochemical functions and drug discovery processes.