The MOL file format, commonly known as an MDL Molfile, is a standard chemical file format used to store information about the atoms, bonds, connectivity, and coordinates of chemical molecules. Developed by Molecular Design Limited (MDL), this format is heavily utilized in cheminformatics and computational chemistry to represent molecular structures in a way that is easily readable by both humans and specialized software. A typical MOL file consists of a header block with metadata, a connection table containing atom lists with their 3D or 2D Cartesian coordinates, and a bond list detailing how the atoms are connected and the type of bonds involved. Because of its standardization and simplicity, it serves as a foundational format for sharing chemical data across various chemical databases, molecular modeling software, and electronic laboratory notebooks.